The availability of arbitrary oligonucleotide sequences through chemical synthesis is one of the cornerstones of biotechnology and a prerequisite for technologies such as PCR 1, DNA-sequencing 2, synthetic biology 3, and CRISPR-Cas9 4. Synthetic oligonucleotides are essential for a range of different areas and millions of oligonucleotides are synthesized daily for use in research laboratories, hospitals, and industry. The vision for this technology is direct integration into DNA synthesisers thereby omitting manual synthesis and storage of phosphoramidites. Sterically hindered as well as redox unstable phosphoramidites are synthesised using this methodology and the subsequent couplings are near-quantitative for all substrates. Here, we report on the development of an on-demand flow synthesis of phosphoramidites from their corresponding alcohols, which is accomplished with short reaction times, near-quantitative yields and without the need of purification before being submitted directly to automated oligonucleotide synthesis. The highly optimised automised chemical oligonucleotide synthesis relies upon phosphoramidites as the phosphate precursors and one of the drawbacks of this technology is the poor bench stability of phosphoramidites. Information including SDS and instructions for use.Automated chemical synthesis of oligonucleotides is of fundamental importance for the production of primers for the polymerase chain reaction (PCR), for oligonucleotide-based drugs, and for numerous other medical and biotechnological applications. You can apply to join Belzona Connect to access additional Check with your local Distributor for more informationįor more information about Belzona 1212 contact us or find your local * All products are subject to regional restrictions. Underwater repairs, ideal for use in splash zones and coastal structures.Long-term repair of offshore structures and marine equipment, including ship hulls, rudders, propellers and ballast tanks without taking the ship out of service.In-situ sealing of oil and fluid leaks on storage tanks, engines, gearboxes, transformers and sumps.Plate bonding, corrosion pit filling and localised repair of wet, sweating or oil contaminated pipes where grit blasting cannot be achieved.Can be machined using conventional tools.Outstanding corrosion and chemical resistance.Reduced health and safety risks as it is solvent-free. Ideal for repair kits and emergency repair due to its versatility in use.Simple 1:1 volume mix ratio makes it easy to mix and apply with no need for specialist tools.Excellent mechanical properties ensuring durability and longevity in service.
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